Download Citation on ResearchGate | Actinobacillus seminis isolated from cattle | The organism commonly referred to as Actinobacillus. Actinobacillus seminis is a well known cause of epididymitis in Australian rams ( Baynes and Simmons, ) and has also been reported as associated with. The main causative agents of ovine infectious epididymitis are Brucella ovis, Actinobacillus seminis, and Histophilus somni [1–3].

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Lymphoid hyperplasia was observed in inguinal and iliac lymph node, as well as in the spleen from both experimental groups. Prior to inoculation, all 20 rams were clinically healthy, without palpable lesions in the testes or epididymides. Nonparametric data testicular consistency and length and width of the tail of epididymes were analyzed by Kruskal Wallis, followed by Dunn test for multiple comparisons.

The values of each point represent the average and standard error. Rabbit antiserum Two New Zealand xctinobacillus weighing 2 kg each were intramuscularly inoculated with A.

Microscopically, bacterial epididymitis initially elicits a neutrophilic inflammation that can be followed by epididymal epithelial hyperplasia or metaplasia, duct obstruction with content retention. In addition, frequency of detection of A.

As Figure 2A shows, Western-blot tests of whole-cell extracts with rabbit hyperimmune serum against A.

The length was measured in dorsoventral and width in craniocaudal direction. Gross findings in rams experimentally infected by Histophilus somni. This disease has been reported in all major sheep producing regions in the world, including Brazil. A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids. Several previous studies evaluated the pathological changes in rams experimentally infected with B.


An invertebrate coagulation system activated by endotoxin: Abstract Actinobacillus seminis is a gram-negative bacterium of the Pasteurellaceae family that is involved in ovine epididymitis. Specific protein of A. According to previous studies, testicular and epididymal changes caused by inoculation of A.

Materials and methods Bacterial strains and growth conditions The bacterial species used in this study are shown in Table I. Interestingly, there was a low frequency of bacterial isolation at the inoculation site, which suggests that the chronicity of infection in the tail of the epididymis may prevent bacterial isolation [ 24 ]. Ovine brucellosis caused by B.

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They contain mainly outer membrane components and associated toxins and enzymes 19 Subclinical cases have been known acfinobacillus persist for up to 4 years. Microscopic changes were similar between the two experimental infections. No protein of these bacteria cross-reacted with the kDa protein of A.

A pattern corresponding to smooth LPS was found in A. The analyses were performed with the GraphPad InStat program, version 3. To detect the kDa protein in membrane microvesicles of A. A, extracts of each bacterium were exposed to antibody against A. The organism has been recovered from placenta and uterus of ewes after natural and experimental infections Al-Katib and Dennis, Looking for a protein specific to this species, we determined the protein profile of subcellular fractions of A.

View at Google Scholar I. Electron microscopy For negative staining, cultured A. For the determination of tissue distribution of agents, fragments of tail, body and head of the epididymes, testicles, ampullae of the vas deferens, seminal vesicles, bulbourethral glands, kidneys, bladder, inguinal and iliac lymph nodes, spleen, and liver were collected. se,inis


Molecular aspects of some virulence factors of Haemophilus sommus. They also provide an excellent source for the purification and characterization of biologically active molecules. Experimental infections were conducted actibobacillus and the experimental groups never had contact. The bacterial isolate was catalase positive and oxidase negative. On the other hand, antibodies against the other bacteria did not recognize the kDa protein in whole-cell extracts Figure 2B.

Identification of an immunogenic protein of Actinobacillus seminis that is present in microvesicles

Subscribe to Table of Contents Alerts. The results suggest that the kDa protein could be used to establish a diagnostic test specific for ovine epididymitis caused by A. Traduit par Docteur Serge Messier. All clinical evaluations were carried out by the same examiner.

Gross and microscopic findings in both experimental infections were similar to those seen in cases of natural infection [ actinobaciluls101124 — 26 ].

Length a and width b of the tail of the epididymides of rams experimentally infected with Actinobacillus seminis or Histophilus somni during the course of infections. Received Oct 4; Accepted Aug 3.

Furthermore, these two organisms induce indistinguishable clinical, gross, or microscopic findings. Al-Katib and Dennis, To receive news and publication updates for The Scientific World Journal, enter your email address in the box below.

B, extract from A.